DNA 恒温快速扩增试剂盒（基础型） DNA Isothermal Rapid Amplification Kit（Basic)

产品参数Product parameters：

注 Tips：

1. 高温变性不能有效去除蛋白，可能对产物分析造成影响；High-temperature denaturation cannot effectively produce protein and may affect product analysis;

2. 市场上部分扩增产物纯化试剂盒不适用，可能造成假阴性结果。Some amplification product purification kits on the market are not suitable and may cause false negative results.

原理概述 Principle overview：

本试剂盒基于一种常温恒温核酸快速扩增技术：在常温恒温下（一般为39ºC~42ºC），在辅助蛋白和单链结合蛋白的帮助下，重组酶和引物形成复合体；进行同源搜索并结合目的同源域，此时在该同源位置形成D-loop区并开始进行链交换；伴随着重组酶从复合体上解离，聚合酶也结合到引物的3’末端，开始链的延伸。

This kit is based on a room temperature and constant temperature nucleic acid rapid amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-chain binding proteins, the recombinase and primers form a complex; homology search is performed And combines with the target homologous domain, at this time, a D-loop region is formed at the homologous position and strand exchange begins; as the recombinase dissociates from the complex, the polymerase also binds to the 3' end of the primer, starting the chain exchange extend.

引物设计 Primer design：

建议使用长度在 30-35 bp 的引物，引物过短会影响扩增速度和检测灵敏度；引物设计避免形成二级结构而影响扩增；扩增子长度建议在 150-300 bp，通常不超过 500 bp。

It is recommended to use primers with a length of 30-35 bp. Primers that are too short will affect amplification speed and detection sensitivity; primers are designed to avoid the formation of secondary structures that affect amplification; the amplicon length is recommended to be 150-300 bp, usually no more than 500 bp.

由于试剂盒灵敏度非常高，在进行反应时请注意避免核酸污染，并设置空白对照；（图片为凝胶电泳法显示结果）

Since the sensitivity of the kit is very high, please be careful to avoid nucleic acid contamination during the reaction and set a blank control; (The picture shows the results of gel electrophoresis)

产品特点及优势Product features and advantages：

本试剂盒具有灵敏度高、特异性强、反应时间短（仅需 30 min）等优点；反应组分为干粉状态，操作简便，易于保存。使用金属浴、水浴锅等即可进行反应操作；无需购买 PCR 仪等价格高昂的设备。

This kit has the advantages of high sensitivity, strong specificity, and short reaction time (only 30 minutes). The reaction components are in dry powder state, which is easy to operate and easy to store. Reaction operations can be performed using metal baths, water baths, etc.; there is no need to purchase expensive equipment such as PCR machines.

产品用途Application：

适用于实验室级别的DNA扩增以及其他检测用途的DNA扩增使用。

Suitable for laboratory-grade DNA amplification and DNA amplification for other detection purposes.

操作步骤 Operation procedure and process：

实验操作相关视频，请微信扫码联系K8凯发国际生物客服获取。

For videos related to experimental operations, please scan the QR code on WeChat to contact customer service.

产品线Product Line：

性能对比数据Performance comparison data：

Q：如何提高结果准确度？

A：使用前请仔细阅读说明书，严格按照操作步骤进行操作；

Please read the instruction manual carefully before use and strictly follow the operating steps.

Q：如何降低实验室污染，避免出现假阳性？

A：样品处理应该严格按照生物安全规范操作，在进行反应时请注意避免核酸污染，并设置空白对照；

Sample processing should be done in strict accordance with biosafety regulations. Please be careful to avoid nucleic acid contamination when performing reactions and set up blank controls.

Q：不能一次性使用完所有试剂，应该如何处理？

A：使用时请取出实验所需的冻干试剂数量，剩余部分请置于存储条件下，注意盖子密封好；

 When using, please take out the amount of freeze-dried reagent required for the experiment, and keep the remaining part under storage conditions. Make sure the lid is tightly sealed;

Q：试剂和组分不同批次是否可以混着用？

A：使用有效期内试剂，且各组分不得与其他批号的相应成分混用。

Use reagents within the validity period, and each component must not be mixed with corresponding components from other batches.