快速核酸释放剂（DNA型）-II型    Rapid Nucleic Acid Release Reagent (DNA) - Type II

产品参数Product parameters：

产品特点及优势Product features and advantages：

产品用途Application：

用于拭子类样本、植物组织类样本的预处理和核酸释放，快速裂解各类常见样本，无需纯化；样本裂解物可直接作为模板进行扩增反应。

It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.

Tips：其下游结合我司恒温扩增试剂对核酸进行检测。

Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.

操作步骤 Operation procedure and process：

性能对比数据Performance comparison data：

关于K8凯发国际 About us：

K8凯发国际生物是集恒温快速核酸扩增底层技术及成品试剂的研发、生产、订制和销售于一体的高新技术企业。

Amp- Future Biotech is a high-tech enterprise integrating R&D, production, customization and sales of the underlying technology of isothermal rapid nucleic acid amplification and finished reagents.

团队历经十多年的技术沉淀，开发出代表未来生物检测技术趋势的多酶恒温快速扩增技术MIRA（Multienzyme Isothermal Rapid Amplification）。并以MIRA为核心平台技术研发出“超快速核酸释放技术”、“自测型核酸检测装置”、“小型自动化检测系统”、“双通道恒温荧光检测仪”、“一体化全自动封闭检测系统”等一系列配套产品，力求打造出快速分子检测领域的整体解决方案，将MIRA技术的灵敏度高、特异性强、快速简便、污染风险小、现场作业等特点充分体现出来。

After more than ten years of technical precipitation, the team developed the Multienzyme Isothermal Rapid Amplification technology MIRA (Multienzyme Isothermal Rapid Amplification), which represents the trend of future biological detection technology. With MIRA as the core platform technology, we have developed a series of supporting products such as "ultra-fast nucleic acid release technology", "self-detection nucleic acid detection device", "small automatic detection system", "double-channel isothermal fluorescence detector", "integrated automatic closed detection system", and strive to create an overall solution in the field of rapid molecular detection. The characteristics of MIRA technology, such as high sensitivity, strong specificity, fast and simple, low pollution risk and field operation, are fully demonstrated.

科研客户涉及各大高校的生命科学学院、医学院、生物工程学院、农学院、动物学院、水产学院等等，还与疾病预防控制中心、微生物研究所、农业科学院、海关等达成合作。目前已触达海内外多个地区的科研市场。据不完全统计，已有35+篇SCI文章，还有不少科研转产项目进行中。

Scientific research customers involve the college of life sciences, medical college, biological engineering college, College of Agriculture, College of Zoology, College of Fisheries, etc., and also cooperate with the Center for Disease Control and Prevention, Microbiology Institute, Academy of Agricultural Sciences, Customs and so on. At present, it has reached the scientific research market in many regions at home and abroad. According to incomplete statistics, there have been 35+ SCI articles, and there are many scientific research conversion projects in progress.

K8凯发国际将继续发挥诊断生态的优势，链接更多海内外科研机构和IVD企业，持续推进，深度融合，促进商机转化，让MIRA技术精准快速诊断惠及更多人，守护造福人类生命健康。

In the future, Amp-future will continue to give play to the advantages of diagnostic ecology, link more scientific research institutions and IVD enterprises at home and abroad, continue to promote, deep integration, and promote the transformation of business opportunities, so that MIRA technology accurate and rapid diagnosis can benefit more people, protect and benefit human life and health.

Q：裂解效果差是怎么回事？

A：样本须与裂解液充分混合，可用枪头吹吸或振荡混匀，提高裂解效率。

In order to ensure the cracking effect, the sample must be fully mixed with the cracking liquid, which can be blown or mixed by the gun head or by oscillation to improve the cracking efficiency.

Q：LD-1管结晶凝固了怎么办？

A：LD-1 在温度低于20 ℃环境下易结晶或凝固，使用前可用 30 ℃- 40 ℃水浴或金属浴仪器进行融解。

LD-1 is easy to crystallize or solidify at temperatures below 20 ℃, and can be melted by 30-40 ℃water bath or metal bath instrument before use.

Q：上清液是否可以作为模板，直接用于扩增反应？

A：涡旋或轻弹振荡混匀后取裂解物上清液作为模板，直接用于扩增反应。

The supernatant of  lysate was used as the template for the amplification reaction after mixing with vortex or flick oscillation.

Q：组织/固体样本如何处理，获得裂解液呢？

A：组织/固体样本裂解后的乳浊液或悬浊液可用离心机短暂离心后取上清液作为模板。

The emulsion or suspension of the tissue/solid sample after cracking can be centrifuged for a short time and then the supernatant can be taken as a template.

Q：快速核酸释放剂（DNA型）-II型是否需要冷链运输？

A：运输条件：常温运输；

Transport conditions: normal temperature transport;

Q：快速核酸释放剂（DNA型）-II型的保存温度是什么？

A：储存条件：常温保存；

Storage conditions: room temperature storage; 

Q：快速核酸释放剂（DNA型）-II型产品有效期多久？

A：产品有效期：12 个月；

Product validity: 12 months; 

Q：快速核酸释放剂（DNA型）-II型的生产日期位置？

A：生产日期见外包装；

Production date is on the outer package

Q：快速核酸释放剂（DNA型）-II型是否需要恒温加热？

A：混匀后置于40°C孵育5min，用于上样检测；

After mixing, incubate at 40°C for 5min for sample testing;